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BIOTECHNOLOGY PRODUCTS DEVELOPED

Genetic Engineering of chickpea for insect resistant (IR) trait Technologies:

Sl No. Title Biotechnological Product-1
1 (a) Name of the Division Division of Plant Biotechnology
1 (b) Name of the Institute ICAR-Indian Institute of Pulses Research, Kanpur 208 024
2. Title of the research and technologies developed during last five years Genetic Engineering of chickpea for insect resistant (IR) trait

Technologies:

  • In vitro regeneration of chickpea using direct organogenetic pathway
  • Indirect method of gene transfer in chickpea tissues using Agrobacterium tumefaciens
  • Direct method of gene transfer in chickpea tissues using particle bombardment
3. Salient features of the research/technology
  • Induction and development of multiple shoots from embryonic axis explants of chickpea using phytohormones combination
  • Development of grafting techniques for establishment of in vitro regenerated shoots into mature fertile plants
  • Agrobacterium tumefaciens mediated genetic transformation of chickpea (Cry, Vip genes)
  • Techniques to detect the presence and expression of transgene across generations
  • Modified particle bombardment method for enhanced transformation frequency of chickpea
4. Developed by  (Name/Designation/Contact No./Email ID) Dr ALOK DAS, Senior Scientist, Division of Plant Biotechnology, ICAR-Indian Institute of Pulses Research, Kanpur 208 024

P: 0512-2582580 EXT 1006; M: 9451446964

E: alok.das@icar.gov.in
5. How this technology contributed to Agricultural advancements or improved farmers practices Chickpeas were thought recalcitrant to regeneration and transformation. Development of routine protocol for regeneration amenable to genetic transformation (direct and indirect) opens up the possibility to transfer/edit genes in chickpea. Transfer of AtDREB1a gene for enhanced drought tolerance was also demonstrated using the technology. The technology provides an important tool for functional as well as translational genomic studies in chickpea

 

Genetic Engineering of pigeonpea for insect resistant (IR) trait

Sl No. Title Biotechnological Product-2
1 (a) Name of the Division Division of Plant Biotechnology
1 (b) Name of the Institute ICAR-Indian Institute of Pulses Research, Kanpur 208 024
2. Title of the research and technologies developed during last five years Genetic Engineering of pigeonpea for insect resistant (IR) trait

Technologies:

  • In vitro regeneration of pigeonpea using direct organogenetic pathway
  • Indirect method of gene transfer in pigeonpea tissues using Agrobacterium tumefaciens
  • Method to screen transgenic pigeonpea seeds using lateral root inhibition (LRI) technology
3. Salient features of the research/technology
  • Induction and development of multiple shoots from embryonic axis explants of pigeonpea using phytohormones combination
  • Development of grafting techniques for establishment of in vitro regenerated shoots into mature fertile plants
  • Agrobacterium tumefaciens mediated genetic transformation of pigeonpea (Cry genes)
  • Techniques to detect the presence and expression of transgene across generations
  • Use of kanamycin to screen transgenic pigeonpea seeds by inhibiting lateral roots
4. Developed by  (Name/Designation/Contact No.Email ID) Dr ALOK DAS, Senior Scientist, Division of Plant Biotechnology, ICAR-Indian Institute of Pulses Research, Kanpur 208 024

P: 0512-2582580 EXT 1006; M: 9451446964

E: alok.das@icar.gov.in
5. How this technology contributed to Agricultural advancements or improved farmers practices Pigeonpea were thought recalcitrant to regeneration and transformation. Development of routine protocol for regeneration amenable to genetic transformation opens up the possibility to transfer/edit genes in pigeonpea. The technology provides an important tool for functional as well as translational genomic studies in pigeonpea

 

Genome Editing (SDN1) of chickpea for yield attributing traits

Sl No. Title Biotechnological Product-3
1 (a) Name of the Division Division of Plant Biotechnology
1 (b) Name of the Institute ICAR-Indian Institute of Pulses Research, Kanpur 208 024
2. Title of the research and technologies developed during last five years Genome Editing (SDN1) of chickpea for yield attributing traits

Technologies:

  • Optimization of genome editing reagents
  • Design and synthesis of guide RNA for targeted mutation
  • Method to screen and identify mutants in the target gene/locus
3. Salient features of the research/technology
  • Fine tuning regulatory elements and combinations thereof for expression in chickpea tissues
  • Customized InFusion cloning technology for cloning and sub-cloning of regulators elements
  • Identification of best possible gRNA using in vitro transcription assay
  • Identification of target mutants using PCR based assays
4. Developed by  (Name/Designation/Contact No.Email ID) Dr ALOK DAS, Senior Scientist, Division of Plant Biotechnology, ICAR-Indian Institute of Pulses Research, Kanpur 208 024

P: 0512-2582580 EXT 1006; M: 9451446964

E: alok.das@icar.gov.in
5. How this technology contributed to Agricultural advancements or improved farmers practices Genome Editing permits targeted mutation of gene in precise manner. Development of the technology shall enable gene editing of chickpea for target traits of Agricultural Importance

 

Speed Breeding protocols for rapid generation advancement of chickpea:

Sl No. Title Biotechnological Product-4
1 (a) Name of the Division Division of Plant Biotechnology
1 (b) Name of the Institute ICAR-Indian Institute of Pulses Research, Kanpur 208 024
2. Title of the research and technologies developed during last five years Speed Breeding protocols for rapid generation advancement of chickpea:

  • Optimization of recipe of the matrix for speed breeding protocol
  • Light simulation for faster seed to seed cycle
  • Temperature and humidity regimes during the entire cycle
  • In vitro culture of green seeds to develop mature fertile plants
3. Salient features of the research/technology
  • Matrix comprising of coco peat, vermicompost, soil and slow release fertilizer supports plants growth and development to maturity
  • RED light and white light spectrum in combination accelerated growth and development with maximum seed set in chickpea (20:4 light and dark phase)
  • Temperature regimes of 22°C and humidity 70% augments the growth of the chickpea plants to maturity
  • Green seeds (20-24 days post anthesis) can be harvested and raised in plant tissue culture
  • Developed technologies can reduce seed to seed cycle to 60 -75 days, across chickpea genotypes tested
4. Developed by  (Name/Designation/Contact No.Email ID) Dr ALOK DAS, Senior Scientist, Division of Plant Biotechnology, ICAR-Indian Institute of Pulses Research, Kanpur 208 024

P: 0512-2582580 EXT 1006; M: 9451446964

E: alok.das@icar.gov.in
5. How this technology contributed to Agricultural advancements or improved farmers practices Speed Breeding can accelerate the generation advancement scheme in chickpea breeding. This in turn can lead to quicker development of genome edited lines, mapping populations and development of chickpea varieties.

MICROBIAL CONSORTIUM

Microbial consortium to mitigate moisture deficit stress

S. No. Title Microbial consortium
1 (a) Name of Division: Division of Basic Sciences
  (b) Name of Institute: ICAR-Indian Institute of Pulses Research, Kanpur
2. Title of the Research & Technologies developed during the last year five years: Microbial consortium for mitigation of moisture deficit stress in pulses 

Developed in 2017 and evaluated under AICRP-Chickpea during 2017-2020
3. Salient feature of the Research/Technology : Microbial consortium consisting bacterial determinants with mechanisms via ACC deaminase, production of plant hormones line cytokinin and IAA, production of extracellular polymeric substances to prevent ethylene stress, and to improve plant growth and water use efficiency through biofilm formation.
4. Developed by (Name/Designation/Contact no./Email Id): Director, ICAR-IIPR, Kanpur (Lead) 

Dr. M.Senthilkumar, Pr. Scientist (Microbiology) (Lead) Mo. 8576063475 Mail. m.kumar@icar.gov.in
5. How this Technology contributed to agricultural advancements or improved farmer practices : Microbial consortium mitigates moisture deficit stress during crop growth stage by increasing water use efficiency through biofilm formation and supports plant growth by producing growth hormones and delay the senescence by balancing ABA/cytokinin ratio in stressed plants. Pooled data of multilocation trails under AICRP-Chickpea (2017-2020) indicated 8% enhanced yield over Mesorhizobium treatment.
6. Details of Impact on farmer’s livelihood and agricultural practices: Microbial consortium is being produced and supplied on demand basis. Bioinoculant production unit is being developed for mass production and supply to farmers. 

Awareness programs were organized to attract entrepreneurs and ABI-incubatees.
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